Shortage of RNA extraction kits threatens coronavirus testing

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second wave

Politico reported today that CDC Director Robert Redfield said on Tuesday that he is not confident that U.S. labs have an adequate stock of the supplies used to extract genetic material from any virus in a patient’s sample — a critical step in coronavirus testing. The article goes on to quote Michael Mina, MD, PhD assistant professor of epidemiology at Harvard’s T.H. Chan School of Public Health, as saying “RNA extraction is the first step in being able to perform” a coronavirus test. “If we cannot perform this step, the [coronavirus] test cannot be performed.”

QIAGEN was cited as a major supplier of RNA purification kits but they are on backorder due to worldwide demand for coronavirus testing. While we know from our own analysis of the market that QIAGEN is indeed a major player in the market there are certainly many other options for labs to turn to.

 

Nucelic Acid Prep Market

The Nucleic Acid Sample Preparation Market

First, a little background. Nucleic acid (NA) sample preparation can be defined as any operation starting with a biological sample and ending in analysis of nucleic acid, namely DNA and/or RNA, that is carried out in a given workflow. This sample preparation step is critical for multiple reasons, including prevention of nucleic acid degradation and minimizing contamination.

Current methods for nucleic acid isolation include column-based purification, and biomagnetic separation. Both methods take advantage of the affinity of silica for nucleic acid materials; column-based methods involve binding NA materials to silica membranes contained in plastic columns, while biomagnetic separation utilizes silica-coated magnetic beads. Since RNA differs structurally from DNA (the former being single-stranded while the latter is double-stranded), the pH and salt concentration of the binding solution can be optimized for binding of one or the other. In this way, RNA preparation kits specifically target RNA molecules for isolation.

For RNA purification using columns, cells are first lysed to release nucleic acid contents, and the lysate mixture, containing RNA binding buffers, is passed through the column. RNA remains bound in the column while other materials are washed away. Elution yields purified RNA. When done manually, liquids are forced through the columns by centrifugation. Alternatively, a vacuum manifold can be used to draw liquids through. Automated column extraction incorporates liquid handling robots and vacuum manifolds, allowing high-throughput preparation of RNA samples.

Biomagnetic separation technology is a more recently developed method for nucleic acid purification. The process, which is gentler than other methods, involves moving the liquid sample through silica-coated beads, much like liquid chromatography. By agitation, a suspension of these magnetic beads is thoroughly mixed with a lysate mixture containing the RNA and binding buffer. A magnet is then used to immobilize the magnetic particles and trap the RNA, while the unbound materials are removed by aspiration. The bound RNA molecules are detached from the beads, washed, and collected for subsequent analysis or further manipulation. Biomagnetic bead separation offers several advantages in that it subjects the analytes to very little mechanical stress, produces higher purity, and can increase product yields. This makes biomagnetic separation more suitable when isolating longer targets, or if secondary or even tertiary structures need to be preserved.

The suppliers of RNA purification kits are numerous and include both large and small companies. Let’s hope they can move quickly to help meet the growing demand.

RNA Kit Suppliers